2 research outputs found
Biogeography and Phylogeny of Aigarchaeota, A Novel Phylum of Archaea
‘Aigarchaeota’ is a candidate phylum of Archaea known only by 16S rRNA gene fragments from cultivaÂtion-independent microbial surveys and a single composite genome from Candidatus ‘Caldiarchaeum subÂterraneum’, an inhabitant of a subterranean gold mine in Japan. Gene sequences associated with ‘AigarchaeoÂta’ have been found in a variety of geothermal habits, however a comprehensive analysis of the phylogeny and distribution of ‘Aigarchaeota’ has not yet been done. Public databases were mined for 16S rRNA gene sequences related to known ‘Aigarchaeota’ and a combination of approaches were used to rigorously define the phylogenetic boundaries of the phylum, investigate its distribution, and design potential ‘Aigarchaeota’- specific primers. Approximately 300 16S rRNA genes and gene fragments affiliated with ‘Aigarchaeota’ were identified, phylogenic analyses suggested that ‘Aigarchaeota’ belonged to at least three family- to order-level groups and at least 13 genus-level groups, and supported the proposed relationship between ‘Aigarchaeota’, Thaumarchaeota, Crenarchaeota, and Korarchaeota in the so-called ‘TACK’ superphylum. A global distriÂbution was suggested with genus-level group habitat differentiation. Some groups were predominantly terÂrestrial (1A, 2B, 3, 4 & 5) while other groups were mostly found in Marine habitats (6-8). Fifteen strong ‘AiÂgarchaeota’-specific primer candidates for the polymerase chain reaction (PCR) amplification of 16S rRNA genes were designed for further analyses
McNair Research Journal - Summer 2015
Journal articles based on research conducted by undergraduate students in the McNair Scholars Program
Table of Contents
Biography of Dr. Ronald E. McNair
Statements:
Dr. Neal J. Smatresk, UNLV President
Dr. Juanita P. Fain, Vice President of Student Affairs
Dr. William W. Sullivan, Associate Vice President for Retention and Outreach
Mr. Keith Rogers, Deputy Executive Director of the Center for Academic Enrichment and Outreach
McNair Scholars Institute Staf